Codon Optimization and Gene Synthesis

The same protein sequence expressed from a murine antibody cDNA and from a human codon-optimized synthetic gene can differ by 10–20 fold in expression level in a human-derived host cell. Codon optimization is not a formality but a quantitative engineering decision with direct consequences for successful biologic production. This module covers the biology behind codon bias, the algorithms used to exploit it, and the gene synthesis platforms that turn a designed sequence into physical DNA.

── Module 07 ・ Deep Engineering (10 lessons)